6NQK
Crystal structure of fast switching M159T mutant of fluorescent protein Dronpa (Dronpa2), Y63(3-FY)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.195 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.750, 86.480, 143.810 |
| Unit cell angles | 90.00, 94.89, 90.00 |
Refinement procedure
| Resolution | 39.756 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uts |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.978 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13RC2_2986: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.756 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.092 | 0.860 |
| Number of reflections | 134566 | 9849 |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 10.9 | 4.2 |
| CC(1/2) | 0.999 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.1 M Tris-HCl at pH 7.4, 0.1 M MgCl2, 12% PEG 3350 |
