6NQ7
Crystal structure of YetJ from Bacillus Subtilis crystallized in lipidic cubic phase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.864, 63.037, 47.336 |
| Unit cell angles | 90.00, 93.95, 90.00 |
Refinement procedure
| Resolution | 47.220 - 2.500 |
| R-factor | 0.24347 |
| Rwork | 0.243 |
| R-free | 0.26025 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pgr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.986 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.220 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 6634 | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 95.3 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8 | 298 | 0.1 M Tris, pH 7.8, 5% PGA-LM (w/v), and 30% PEG 400 (v/v) |
