6NQ6
Structure & function of a new Aspartylglucosaminuria variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-28 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.150, 96.020, 61.490 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.500 |
| R-factor | 0.19582 |
| Rwork | 0.194 |
| R-free | 0.22446 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gl9 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.214 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 80277 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.0 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.2M NaCl, 0.1M HEPES (pH 7.5), 27% PEG 3350 |
