6NP9

PD-L1 IgV domain V76T with fragment

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-16
Detector	MAR CCD 165 mm
Wavelength(s)	1
Spacegroup name	C 2 2 21
格子定数 [Å]	53.331, 54.111, 85.724
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	28.427 - 1.270
R因子	0.204
Rwork	0.202
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bik
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	0.895
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	40.000	1.290
分解能 [Å] (高)	1.270	1.270
Rmerge_l_obs	0.055	0.288
Rpim	0.018	0.112
独立反射数	32928	1604
<I/σ(I)>	61	7.1
完全性 [%]	99.6	99.8
冗長性	9.2	7.1
CC(1/2)		0.937

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	EVAPORATION		291	Ammonium Sulfate