6NOT

Crystal structure of a full length elongation factor G (EF-G) from Rickettsia prowazekii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 1
Unit cell lengths	57.670, 79.560, 95.470
Unit cell angles	103.85, 95.32, 92.17

Refinement procedure

Resolution	32.597 - 2.400
R-factor	0.1995
Rwork	0.197
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4juw
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((dev_3374))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.597	32.597	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.040	0.028	0.596
Rmeas	0.047	0.034	0.712
Total number of observations	206687
Number of reflections	62945	652	4651
<I/σ(I)>	15.9	34.97	2.05
Completeness [%]	98.2	92	97.5
Redundancy	3.284	2.916	3.343
CC(1/2)	0.999	0.998	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	287	RiprA.18013.a.B1.PW38427 at 10.98 mg/mL against MCSG1 screen condition H6: 0.1 M sodium acetate pH 4.6, 3.5 M sodium formate with direct cryo, crystal tracking ID 299862h6, unique puck ID pxh5-7