6NOS
PD-L1 IgV domain V76T with fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-28 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.014, 97.401, 33.182 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.421 - 2.701 |
R-factor | 0.1944 |
Rwork | 0.185 |
R-free | 0.27540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i8l |
RMSD bond length | 0.010 |
RMSD bond angle | 1.415 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.129 | 0.469 |
Rmeas | 0.146 | 0.566 |
Rpim | 0.066 | 0.309 |
Number of reflections | 7548 | 354 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 94.2 | 89.6 |
Redundancy | 4.1 | 2.6 |
CC(1/2) | 0.079 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 291 | 0.056 M sodium phosphate monobasic, 1.344 M potassium phosphate dibasic |