6NOQ
Xanthomonas citri Phospho-PGM in complex with mannose-1-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2018-01-31 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.700, 54.640, 173.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.253 - 1.610 |
R-factor | 0.2102 |
Rwork | 0.207 |
R-free | 0.27070 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.884 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.253 | 1.640 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.311 | 1.784 |
Rmeas | 0.324 | 1.904 |
Rpim | 0.092 | 0.652 |
Number of reflections | 55020 | 2656 |
<I/σ(I)> | 7 | 0.7 |
Completeness [%] | 100.0 | 99.6 |
Redundancy | 12.1 | 8.2 |
CC(1/2) | 0.991 | 0.348 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 22% PEG 8000, 0.2M MgCl, 0.1M HEPES |