6NOI
Crystal structure of Tsn15 in apo form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.838, 36.938, 91.815 |
| Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
| Resolution | 29.699 - 1.800 |
| R-factor | 0.1571 |
| Rwork | 0.156 |
| R-free | 0.18640 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.885 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.700 | 29.700 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
| Rmerge | 0.063 | 0.052 | 0.218 |
| Rmeas | 0.068 | 0.056 | 0.253 |
| Rpim | 0.027 | 0.022 | 0.125 |
| Total number of observations | 239481 | 1982 | 7862 |
| Number of reflections | 38365 | 341 | 2014 |
| <I/σ(I)> | 22.7 | 40.6 | 6.4 |
| Completeness [%] | 99.0 | 96.8 | 88.7 |
| Redundancy | 6.2 | 5.8 | 3.9 |
| CC(1/2) | 0.998 | 0.998 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.1 M PCTP pH 6, 25% PEG 1500 buffer |
