6NOH

Crystal structure of FBF-2 repeat 5 mutant (C363S, R364Y, Q367S) in complex with 8-nt RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-14
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	96.397, 96.397, 99.853
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.676 - 2.249
R-factor	0.1625
Rwork	0.159
R-free	0.20560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k5q
RMSD bond length	0.007
RMSD bond angle	0.794
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.250	6.100	2.250
Rmerge	0.101	0.050	0.704
Rmeas	0.112	0.055	0.776
Rpim	0.047	0.023	0.323
Total number of observations	142174
Number of reflections	25098	1291	1266
<I/σ(I)>	9.3
Completeness [%]	99.9	99.2	100
Redundancy	5.7	5.3	5.7
CC(1/2)		0.997	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Tris pH 8.6, 15% [w/v] PEG 8000, 8% [v/v] ethylene glycol