6NOF
Crystal structure of FBF-2 repeat 5 mutant (C363A, R364Y, Q367S) in complex with 8-nt RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-14 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 96.544, 96.544, 101.066 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.211 - 2.251 |
R-factor | 0.1685 |
Rwork | 0.164 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k5q |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 6.100 | 2.250 |
Rmerge | 0.104 | 0.043 | 0.772 |
Rmeas | 0.115 | 0.047 | 0.848 |
Rpim | 0.048 | 0.020 | 0.350 |
Number of reflections | 25386 | 1291 | 1266 |
<I/σ(I)> | 8.6 | ||
Completeness [%] | 99.9 | 98.5 | 100 |
Redundancy | 5.7 | 5.5 | 5.7 |
CC(1/2) | 0.998 | 0.802 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM Tris pH 8.0, 15% [w/v] PEG 8000, 8% [v/v] ethylene glycol |