6NOB

Structure of Glycoside Hydrolase family 32 from Bifidobacterium adolescentis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-06
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.45866
Spacegroup name	P 31 2 1
Unit cell lengths	130.903, 130.903, 132.937
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.140 - 2.440
R-factor	0.1783
Rwork	0.177
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uyp
RMSD bond length	0.002
RMSD bond angle	0.574
Data reduction software	TRUNCATE
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.340	2.500
High resolution limit [Å]	2.440	2.440
Rmerge	0.282	3.425
Rmeas	0.298	3.670
Rpim		0.926
Number of reflections	49401	54042
<I/σ(I)>	9.9	1
Completeness [%]	100.0	100
Redundancy	18.4	15.1
CC(1/2)	0.996	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	PEG 8K 25% (w/v), MES 0.1M pH 6.5