6NO0

ADP bound to ATP-grasp fold of Blastocystis hominis succinyl-CoA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	C 2 2 21
Unit cell lengths	92.203, 116.981, 127.161
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.323 - 2.209
R-factor	0.2138
Rwork	0.213
R-free	0.23870
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.050	43.050	2.270
High resolution limit [Å]	2.209	9.880	2.210
Rmerge	0.067	0.034	0.852
Number of reflections	34802
<I/σ(I)>	12.3
Completeness [%]	100.0	98.8	100
Redundancy	6.5	6	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	10% w/v PEG3350, 100 mM MES, pH 5.7, 160 mM ammonium tartrate, pH 8