6NNX
Crystal Structure of the All-Trans Retinal-Bound R111K:Y134F:T54V:R132Q:P39Y:R59Y:L121M Mutant of Human Cellular Retinoic Acid Binding Protein II in the Dark at 1.87 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-18 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97872 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 58.842, 58.842, 99.535 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.360 - 1.870 |
R-factor | 0.2107 |
Rwork | 0.208 |
R-free | 0.24040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yfp |
RMSD bond length | 0.008 |
RMSD bond angle | 0.969 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.360 | 1.937 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.090 | 0.660 |
Number of reflections | 16795 | 1647 |
<I/σ(I)> | 20.9 | 2.4 |
Completeness [%] | 98.3 | 98.86 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350, Sodium Malonate |