6NNG

Tubulin-RB3_SLD-TTL in complex with compound DJ95

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	105.418, 157.979, 182.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.253 - 2.397
R-factor	0.1938
Rwork	0.192
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	HKL-2000
Refinement software	PHENIX (1.13_2998: ???)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.397	2.397
Number of reflections	120100
<I/σ(I)>	20.89
Completeness [%]	99.2
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	6% PEG 4000, 5% Glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2