6NMY
A Cytokine-receptor complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.95370 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 111.647, 157.251, 168.306 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.760 | 47.760 | 3.420 |
High resolution limit [Å] | 3.300 | 12.780 | 3.300 |
Rmerge | 0.175 | 0.027 | 1.728 |
Rmeas | 0.194 | 0.030 | 1.914 |
Rpim | 0.081 | 0.013 | 0.807 |
Total number of observations | 248002 | 3883 | 24488 |
Number of reflections | 45227 | 836 | 4368 |
<I/σ(I)> | 8.8 | 41.1 | 1.1 |
Completeness [%] | 99.8 | 95 | 99.5 |
Redundancy | 5.5 | 4.6 | 5.6 |
CC(1/2) | 0.996 | 0.999 | 0.310 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277.15 | Crystals were grown in 10 mM Tris pH6.8, 8% PEG 8000, 0.15 M magnesium chloride |