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6NMY

A Cytokine-receptor complex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron siteAustralian Synchrotron
BeamlineMX2
Temperature [K]100
Detector technologyCCD
Collection date2014-06-14
DetectorADSC QUANTUM 315
Wavelength(s)0.95370
Spacegroup nameP 21 21 21
Unit cell lengths111.647, 157.251, 168.306
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution47.448 - 3.301
R-factor0.2384
Rwork0.236
R-free0.27780
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4nkq 5uv8
Data reduction softwareXDS
Data scaling softwareAimless (0.5.15)
Phasing softwarePHASER (2.5.6)
Refinement softwarePHENIX
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]47.76047.7603.420
High resolution limit [Å]3.30012.7803.300
Rmerge0.1750.0271.728
Rmeas0.1940.0301.914
Rpim0.0810.0130.807
Total number of observations248002388324488
Number of reflections452278364368
<I/σ(I)>8.841.11.1
Completeness [%]99.89599.5
Redundancy5.54.65.6
CC(1/2)0.9960.9990.310
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.8277.15Crystals were grown in 10 mM Tris pH6.8, 8% PEG 8000, 0.15 M magnesium chloride

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