6NMS

Blocking Fab 136 anti-SIRP-alpha antibody in complex with SIRP-alpha Variant 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	81.390, 75.190, 103.820
Unit cell angles	90.00, 104.43, 90.00

Refinement procedure

Resolution	50.272 - 2.110
R-factor	0.2063
Rwork	0.204
R-free	0.24380
RMSD bond length	0.007
RMSD bond angle	1.084
Data reduction software	HKL-2000
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.272	2.160
High resolution limit [Å]	2.110	2.110
Number of reflections	68779	5042
<I/σ(I)>	8.7
Completeness [%]	98.4	97.8
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	285	0.1 M Tris-HCl, 0.2 M MgCl2, 15% w/v PEG 4000