6NMS
Blocking Fab 136 anti-SIRP-alpha antibody in complex with SIRP-alpha Variant 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-25 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.390, 75.190, 103.820 |
Unit cell angles | 90.00, 104.43, 90.00 |
Refinement procedure
Resolution | 50.272 - 2.110 |
R-factor | 0.2063 |
Rwork | 0.204 |
R-free | 0.24380 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.084 |
Data reduction software | HKL-2000 |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.272 | 2.160 |
High resolution limit [Å] | 2.110 | 2.110 |
Number of reflections | 68779 | 5042 |
<I/σ(I)> | 8.7 | |
Completeness [%] | 98.4 | 97.8 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 285 | 0.1 M Tris-HCl, 0.2 M MgCl2, 15% w/v PEG 4000 |