6NLY

Fragment of human mitochondrial Alanyl-tRNA Synthetase C-Ala domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97938
Spacegroup name	C 2 2 21
Unit cell lengths	103.220, 317.230, 54.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.019 - 2.307
R-factor	0.2118
Rwork	0.209
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nlq
RMSD bond length	0.011
RMSD bond angle	1.415
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.019	38.019	2.370
High resolution limit [Å]	2.310	10.320	2.310
Rmerge	0.045	0.019	0.732
Rmeas	0.049	0.021	0.785
Total number of observations	291673
Number of reflections	40004	518	2885
<I/σ(I)>	23.99	66.53	3.57
Completeness [%]	99.3	96.5	98.3
Redundancy	7.291	6.608	7.493
CC(1/2)	1.000	1.000	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	298	0.1 M Tris-HCl, 40% PEG200