6NLX
Crystal Structure of Glyceraldehyde-3-phosphate Dehydrogenase from Naegleria fowleri with bound NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-18 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.010, 119.940, 162.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.467 - 1.800 |
R-factor | 0.1691 |
Rwork | 0.169 |
R-free | 0.20390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u8f |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.467 | 49.467 | 1.850 |
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.076 | 0.026 | 0.548 |
Rmeas | 0.084 | 0.029 | 0.600 |
Total number of observations | 760791 | ||
Number of reflections | 129448 | 1508 | 9616 |
<I/σ(I)> | 13.9 | 31.9 | 2.88 |
Completeness [%] | 97.4 | 91.2 | 98.6 |
Redundancy | 5.877 | 5.904 | 6.268 |
CC(1/2) | 0.998 | 0.999 | 0.835 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | MCSG1 F2 (293453f2): 200mM Ammonium Acetate, 100mM Bis-Tris:HCl pH 6.5, 25% (w/v) PEG 3350: NafoA.00855.a.B1.PS38303 @ 21.4mg/mL: 15%EG: zzl0-7: aps_20170818_21idf |