6NLP
The crystal structure of an ABC transporter periplasmic binding protein YdcS from Escherichia coli BW25113
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97943 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.895, 42.896, 122.771 |
| Unit cell angles | 90.00, 121.96, 90.00 |
Refinement procedure
| Resolution | 40.908 - 1.900 |
| R-factor | 0.1829 |
| Rwork | 0.181 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.926 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.908 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.108 | 0.773 |
| Rmeas | 0.105 | 0.935 |
| Rpim | 0.055 | 0.516 |
| Number of reflections | 56396 | 2719 |
| <I/σ(I)> | 11.4 | 1.47 |
| Completeness [%] | 98.4 | 95.4 |
| Redundancy | 3.5 | 3.1 |
| CC(1/2) | 0.487 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2M NaCl, 0.1M HEPES, 25% PEG3350, 1% Trehalose |
