6NKS
Ternary complex crystal structure of K289M variant of DNA polymerase Beta with beta-gamma CHF analog of dGTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-21 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.607, 80.327, 55.391 |
Unit cell angles | 90.00, 108.13, 90.00 |
Refinement procedure
Resolution | 29.269 - 2.349 |
R-factor | 0.1954 |
Rwork | 0.189 |
R-free | 0.25530 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.065 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.349 | 2.349 |
Number of reflections | 25647 | |
<I/σ(I)> | 9 | |
Completeness [%] | 99.8 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 16-18% PEG 3350, 50 mM Imidazole, 350 mM Sodium Acetate |