6NKG
Crystal Structure of the Lipase Lip_vut5 from Goat Rumen metagenome.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-11 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.110, 60.110, 169.898 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.668 - 2.150 |
| R-factor | 0.1967 |
| Rwork | 0.195 |
| R-free | 0.23510 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.404 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.700 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.115 | 0.981 |
| Number of reflections | 17833 | 849 |
| <I/σ(I)> | 13.5 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.9 | 12.7 |
| CC(1/2) | 0.998 | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 289 | 0.2 M magnesium acetate, 20 % PEG3350 |
