6NKF
Crystal Structure of the Lipase Lip_vut4 from Goat Rumen metagenome.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-26 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 157.856, 157.856, 195.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.723 - 2.232 |
| R-factor | 0.1548 |
| Rwork | 0.153 |
| R-free | 0.17910 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.701 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.145 | 0.990 |
| Number of reflections | 119411 | 5862 |
| <I/σ(I)> | 25.4 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 10.1 |
| CC(1/2) | 0.986 | 0.844 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 289 | 0.2 M sodium thiocyanate pH 6.9, 20 % (w/v) PEG3350 |
