6NK8
C-terminal region of the Burkholderia pseudomallei OLD protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 83.256, 105.669, 123.764 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.880 - 2.240 |
R-factor | 0.2159 |
Rwork | 0.214 |
R-free | 0.26050 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.908 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.300 | 2.310 |
High resolution limit [Å] | 2.240 | 2.240 |
Number of reflections | 26567 | |
<I/σ(I)> | 18.2 | |
Completeness [%] | 99.4 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.5, 0.23 M MgCl2, 30% PEG 400 and 0.001 M glutathione |