6NK3
Crystal structure of murine Mxra8 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 293 |
Detector technology | CMOS |
Collection date | 2018-10-05 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.00003 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 72.550, 142.700, 195.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.460 - 2.200 |
R-factor | 0.1997 |
Rwork | 0.199 |
R-free | 0.22580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mj6 |
Data reduction software | XDS (Jan 26, 2018) |
Data scaling software | Aimless (0.6.3) |
Phasing software | PHENIX (1.12) |
Refinement software | PHENIX (1.12) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.460 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 51479 | 4856 |
<I/σ(I)> | 23.63 | 1.21 |
Completeness [%] | 99.4 | 95.83 |
Redundancy | 26.4 | 17.7 |
CC(1/2) | 0.999 | 0.666 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris, pH 8.5, 8% PEG8000, 25% ethylene glycol |