6NJY

Type IV CRISPR associated RNA endonuclease Cas6 - apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	193
Detector technology	PIXEL
Collection date	2018-02-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.55, 1.0718
Spacegroup name	P 61
Unit cell lengths	85.541, 85.541, 142.587
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.004 - 1.760
R-factor	0.1682
Rwork	0.167
R-free	0.19780
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.004	40.000	1.820
High resolution limit [Å]	1.760	3.790	1.760
Rmerge	0.053	0.040	0.727
Rmeas	0.058	0.044	0.822
Rpim	0.023	0.017	0.376
Number of reflections	58149	5922	5618
<I/σ(I)>	18.1
Completeness [%]	99.6	99.9	96.3
Redundancy	6	6.6	4.2
CC(1/2)		0.996	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	Mother Liquor = 0.1 M sodium acetate trihydrate (pH 5.0) and 1.75M ammonium sulfate. 6-8 mg/ml of protein purified in 100 mM HEPES pH 7.5, 150 mM KCl, 5% glycerol, 1mM TCEP, was mixed in equal volumes (2 microliters of each) on a sitting drop pedestal with ~500 mL of mother liquor in reservoir.