6NJX

C-terminal region of the Xanthomonas campestris pv. campestris OLD protein phased with mercury

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.008
Spacegroup name	P 43
Unit cell lengths	65.189, 65.189, 63.799
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.100 - 1.950
R-factor	0.2034
Rwork	0.201
R-free	0.24170
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.190	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	38308
<I/σ(I)>	15.8
Completeness [%]	99.9
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Bis Tris Propane pH 7.0-8.0, 11-26% PEG 3350, and 0.15-0.2 M sodium iodide