6NJK
Crystal structure of beta-lactamase from Sulfitobacter sp. EE-36
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-09 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.694, 107.390, 59.457 |
| Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
| Resolution | 29.948 - 1.550 |
| R-factor | 0.1434 |
| Rwork | 0.143 |
| R-free | 0.17700 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.926 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((dev_2947: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.106 | 1.018 |
| Number of reflections | 98477 | 4838 |
| <I/σ(I)> | 13.7 | 1.65 |
| Completeness [%] | 98.6 | 96.9 |
| Redundancy | 6.6 | 5.7 |
| CC(1/2) | 0.989 | 0.633 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.1 M sodium acetate, 25% PEG4000, 5% 2-propanol, cryo 10% glycerol |
