6NIU

Crystal structure of a human anti-ZIKV-DENV neutralizing antibody MZ4 in complex with ZIKV E glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-15
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97
Spacegroup name	P 1
Unit cell lengths	113.530, 137.260, 136.790
Unit cell angles	80.12, 65.74, 65.75

Refinement procedure

Resolution	9.997 - 4.303
R-factor	0.2689
Rwork	0.264
R-free	0.35900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uta 5ire
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.350
High resolution limit [Å]	4.200	4.200
Rpim	0.230
Number of reflections	16201	2954
<I/σ(I)>	2.64
Completeness [%]	53.2
Redundancy	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	291	1.26 M ammonium sulfate, 0.1 M CHES/NaOH (pH 9.5) and 0.2 M sodium chloride