6NIO
Crystal Structure of the Molybdate Transporter Periplasmic Protein ModA from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-20 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.922, 54.305, 69.785 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.657 - 1.370 |
| R-factor | 0.1611 |
| Rwork | 0.160 |
| R-free | 0.17880 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.234 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.390 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.120 | 0.687 |
| Number of reflections | 41969 | 2078 |
| <I/σ(I)> | 35.9 | 3.25 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 11.9 | 11.6 |
| CC(1/2) | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 289 | 0.1 M CHES pH 9.5, 1.0 M sodium citrate |
