6NIA
Pseudomonas fluorescens isocyanide hydratase at 100 K helical disorder model
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.576, 56.466, 68.233 |
| Unit cell angles | 90.00, 112.49, 90.00 |
Refinement procedure
| Resolution | 37.822 - 1.050 |
| R-factor | 0.1178 |
| Rwork | 0.117 |
| R-free | 0.13420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3non |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.070 | 0.700 |
| Number of reflections | 179412 | 17341 |
| <I/σ(I)> | 22.8 | 2.1 |
| Completeness [%] | 97.1 | 94.2 |
| Redundancy | 6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 24-26% PEG 3350, 200-250 MM MAGNESIUM CHLORIDE, 100 MM TRIS-HCL PH=8.6 |
