6NI5
Pseudomonas fluorescens isocyanide hydratase at 274 K G150A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 274 |
| Detector technology | PIXEL |
| Collection date | 2016-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.82653 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.038, 57.895, 69.110 |
| Unit cell angles | 90.00, 112.55, 90.00 |
Refinement procedure
| Resolution | 33.595 - 1.300 |
| R-factor | 0.1208 |
| Rwork | 0.119 |
| R-free | 0.15190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3non |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.960 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.055 | 1.513 |
| Rmeas | 0.066 | 1.513 |
| Rpim | 0.036 | 0.846 |
| Number of reflections | 100249 | 4568 |
| <I/σ(I)> | 9.1 | 1.2 |
| Completeness [%] | 98.1 | 90.1 |
| Redundancy | 3.3 | 3 |
| CC(1/2) | 0.996 | 0.318 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 25% PEG 3350, 200 mM magnesium chloride, 100mM Tris-HCl, pH 8.6, 2 mM DTT |
