6NHQ
Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin HA2 I45M mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 208.856, 131.006, 72.576 |
Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.19179 |
Rwork | 0.190 |
R-free | 0.21888 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.522 |
Data scaling software | HKL-2000 (712) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.050 | 0.390 |
Number of reflections | 65790 | 7333 |
<I/σ(I)> | 15.4 | 1.5 |
Completeness [%] | 97.6 | 98.4 |
Redundancy | 5.9 | 5.9 |
CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M sodium cacodylate pH 6.5, 5% PEG 8000, and 38% 2-methyl-2,4-pentanediol |