6NFU
Structure of the KcsA-G77A mutant or the 2,4-ion bound configuration of a K+ channel selectivity filter.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-25 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.987 |
Spacegroup name | I 4 |
Unit cell lengths | 155.086, 155.086, 75.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.678 - 2.090 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.22360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k4c |
Data reduction software | HKL-2000 (v1.0) |
Data scaling software | HKL-2000 (v1.0) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.678 | 34.678 |
High resolution limit [Å] | 2.090 | 2.090 |
Number of reflections | 53355 | |
<I/σ(I)> | 30.38 | |
Completeness [%] | 99.9 | |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG400, magnesium acetate, sodium acetate |