6NFS
CopC from Pseudomonas fluorescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.584, 34.753, 94.679 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.650 - 1.500 |
| R-factor | 0.18429 |
| Rwork | 0.183 |
| R-free | 0.21105 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6nfr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.545 |
| Data reduction software | HKL-2000 (5.8.0230) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.650 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 16697 | |
| <I/σ(I)> | 20.7 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.7 M ammonium sulfate, 0.1 M Bis-Tris propane, pH 7.0 |
