6NEZ
Trypanosoma brucei - BDF5, Tb427tmp.01.5000 A, solved with PF-CBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.994, 151.493, 156.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.940 - 2.200 |
| R-factor | 0.2058 |
| Rwork | 0.204 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k29 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.492 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.099 | 0.043 | 0.981 |
| Rmeas | 0.106 | 0.047 | 1.048 |
| Rpim | 0.037 | 0.017 | 0.359 |
| Total number of observations | 286191 | ||
| Number of reflections | 35904 | 1937 | 1743 |
| <I/σ(I)> | 6.3 | ||
| Completeness [%] | 99.6 | 99 | 99.8 |
| Redundancy | 8 | 7.6 | 7.7 |
| CC(1/2) | 0.997 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 2.27 M Na Formate, and 0.1M Na Acetate pH4.5 |
