6NEP

Structure of human PACRG-MEIG1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.476000
Spacegroup name	P 41 21 2
Unit cell lengths	67.371, 67.371, 159.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.638 - 2.098
R-factor	0.22961406499
Rwork	0.229
R-free	0.24933
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.953
Data reduction software	XDS
Data scaling software	Aimless (1.12_2829)
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.640	2.160
High resolution limit [Å]	2.090	2.090
Rmerge	0.093	1.100
Number of reflections	253322	9944
<I/σ(I)>	13.7	1.1
Completeness [%]	99.8	98.4
Redundancy	11.3	5.6
CC(1/2)	0.998	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	18%PEG8K, 60mM KHPO4, 15% Glycerol