6NE4
Designed repeat protein specifically in complex with Fz7CRD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 57.604, 68.367, 86.468 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.578 - 1.648 |
R-factor | 0.1611 |
Rwork | 0.160 |
R-free | 0.18610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5t44 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.329 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.648 | 1.648 |
Rmerge | 0.084 | 0.720 |
Number of reflections | 41862 | 4055 |
<I/σ(I)> | 38.1 | |
Completeness [%] | 99.6 | |
Redundancy | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M Na2HPO4, citric acid, pH 4.2 and 2M Ammonium sulfate |