6NE0

Structure of double-stranded target DNA engaged Csy complex from Pseudomonas aeruginosa (PA-14)

Replaces:  6MPU

EM specimen

Vitrification	Yes
Instrument	HOMEMADE PLUNGER

EM Data Collection

Microscope	FEI TALOS ARCTICA
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Cs (mm)	2.7
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.4
Number of Particle	1543677
Software	RELION,Leginon,CTFFIND
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION