6NDU

Structure of human PACRG-MEIG1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97934
Spacegroup name	P 41 21 2
Unit cell lengths	67.297, 67.297, 158.255
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.586 - 2.100
R-factor	0.1919
Rwork	0.190
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nep
RMSD bond length	0.008
RMSD bond angle	1.077
Data reduction software	XDS (VERSION Nov 1, 2016)
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.9-1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.590	47.590	2.160
High resolution limit [Å]	2.100	8.910	2.100
Rmerge	0.052	0.028	1.303
Rmeas	0.056	0.032	1.411
Rpim	0.022	0.014	0.536
Number of reflections	22042	363	1752
<I/σ(I)>	19.8
Completeness [%]	99.9	99.4	100
Redundancy	6.3	5.1	6.7
CC(1/2)	0.999	0.999	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	18% PEG 8000, 0.04 M KH2PO4, 20% glycerol