6NDN

Crystal Structure of Selenocysteine Lyase from Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96863
Spacegroup name	P 43 21 2
Unit cell lengths	126.520, 126.520, 134.091
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.213 - 1.768
R-factor	0.1605
Rwork	0.160
R-free	0.17270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jf9
RMSD bond length	0.019
RMSD bond angle	1.410
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	92.050	92.050	1.820
High resolution limit [Å]	1.768	7.920	1.770
Rmerge	0.160	0.050	2.354
Rpim	0.032	0.010	0.482
Number of reflections	106193	1374	7721
<I/σ(I)>	11	33.7	1
Completeness [%]	100.0	100	100
Redundancy	25.8	23.4	24.7
CC(1/2)	0.999	0.999	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	4M Sodium formate