6NDI
Crystal Structure of the Sugar Binding Domain of LacI Family Protein from Klebsiella pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-11-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 180.737, 110.478, 37.777 |
Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
Resolution | 29.830 - 2.600 |
R-factor | 0.18447 |
Rwork | 0.182 |
R-free | 0.23513 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.395 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.114 | 0.804 |
Rmeas | 0.124 | 0.875 |
Rpim | 0.049 | 0.342 |
Number of reflections | 22623 | 1122 |
<I/σ(I)> | 19.3 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.3 | 6.4 |
CC(1/2) | 0.867 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | Protein: 3.0 mg/ml, 0.01M Tris-HCl pH 8.3; Screen: Classics II (C1), 3.5M Sodium formate pH 7.0; Cryo: 4.0M Sodium formate. |