6NCY
Crystal structure of hybrid beta-glucuronidase/beta-galacturonidase from Fusicatenibacter saccharivorans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 3 |
Unit cell lengths | 192.562, 192.562, 192.562 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.713 - 2.050 |
R-factor | 0.1543 |
Rwork | 0.154 |
R-free | 0.18000 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.899 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.713 | 2.120 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.168 | 1.711 |
Rpim | 0.028 | 0.460 |
Number of reflections | 147997 | 14676 |
<I/σ(I)> | 21.6 | |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 34.7 | 14.4 |
CC(1/2) | 0.999 | 0.578 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M DL-malic acid, pH 7.0, 20% w/v PEG 3350 |