6NCX
Crystal structure of GH2 beta-galacturonidase from Eisenbergiella tayi bound to galacturonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.342, 156.038, 124.285 |
| Unit cell angles | 90.00, 101.27, 90.00 |
Refinement procedure
| Resolution | 29.877 - 2.250 |
| R-factor | 0.2221 |
| Rwork | 0.221 |
| R-free | 0.27500 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.926 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.880 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.148 | 1.363 |
| Rpim | 0.609 | |
| Number of reflections | 114891 | 65156 |
| <I/σ(I)> | 6.7 | 1.1 |
| Completeness [%] | 99.3 | 96.3 |
| Redundancy | 6.9 | 5.9 |
| CC(1/2) | 0.995 | 0.674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium sulfate, 0.1 M Bis-Tris:HCl, pH 6.5, 25% (w/v) PEG 3350, 1 mM GalA |
