6NCP
Crystal structure of HIV-1 broadly neutralizing antibody ACS202
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 65 |
Unit cell lengths | 78.444, 78.444, 340.854 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 58.300 - 2.760 |
R-factor | 0.2236 |
Rwork | 0.222 |
R-free | 0.25630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zyk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.844 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.310 | 2.810 |
High resolution limit [Å] | 2.760 | 2.760 |
Rmerge | 0.280 | 1.540 |
Rpim | 0.090 | 0.470 |
Number of reflections | 30474 | 1555 |
<I/σ(I)> | 5.4 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.8 | 11.8 |
CC(1/2) | 0.990 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.7 | 293 | 1.6 M ammonium sulfate, 0.1 M bicine, pH8.7 |