6NCE

Crystal structure of the human FOXN3 DNA binding domain in complex with a forkhead DNA sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	.979
Spacegroup name	C 1 2 1
Unit cell lengths	94.940, 102.080, 34.450
Unit cell angles	90.00, 102.65, 90.00

Refinement procedure

Resolution	30.570 - 2.598
Rwork	0.235
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c6y
RMSD bond length	0.004
RMSD bond angle	0.542
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.570	2.691
High resolution limit [Å]	2.598	2.598
Rmerge	0.048	1.328
Rmeas	0.057	1.569
Rpim	0.031	0.825
Number of reflections	9664	958
<I/σ(I)>	12.6	1.09
Completeness [%]	96.6	94.47
Redundancy	3.5	3.5
CC(1/2)	0.996	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	298	0.1 M BisTris pH 5.4, 0.2 M MgCl2, 22% PEG 3350