6NC8
Lipid II flippase MurJ, inward occluded conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.65 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 68.419, 80.261, 100.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.419 - 2.601 |
| R-factor | 0.2302 |
| Rwork | 0.229 |
| R-free | 0.25740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t77 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.625 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 17462 | |
| <I/σ(I)> | 13.29 | |
| Completeness [%] | 99.8 | |
| Redundancy | 48.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5 | 295 | 100 mM Na citrate tribasic-HCl pH 5.0, 40% PEG200 |
