6NC4

FtsY-NG high-resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	34.817, 75.905, 108.403
Unit cell angles	90.00, 93.39, 90.00

Refinement procedure

Resolution	44.059 - 1.600
R-factor	0.1601
Rwork	0.159
R-free	0.18870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.031
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.060	1.600
High resolution limit [Å]	1.600	1.600
Rmerge	0.015	0.111
Rmeas	0.021	0.157
Rpim	0.015	0.111
Number of reflections	74276
<I/σ(I)>	15.92
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.999	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297.15	0.1 M Bis-Tris pH 6.6 , 190 mM Ammonium Acetate, 22% PEG3350