6NBU

CRISPR Complex Subunit Csm2 from Staphylococcus epidermidis RP62a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-09
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	P 41 21 2
Unit cell lengths	77.777, 77.777, 69.472
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.750
R-factor	0.24689
Rwork	0.245
R-free	0.29418
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.689
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.780	2.880
High resolution limit [Å]	2.750	2.750
Number of reflections	5931	765
<I/σ(I)>	22.7	1.8
Completeness [%]	99.9	99.8
Redundancy	6.6	5
CC(1/2)	0.999	0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	283	Tris, ethanol