6NBP
Crystal Structure of a Sugar N-Formyltransferase from the Plant Pathogen Pantoea ananatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-05 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.928, 75.928, 87.591 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.18764 |
| Rwork | 0.186 |
| R-free | 0.22646 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yfv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.554 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.061 | 0.433 |
| Number of reflections | 32264 | 4954 |
| <I/σ(I)> | 13.1 | 2.1 |
| Completeness [%] | 98.3 | 94.1 |
| Redundancy | 7.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 13-16%PEG-3350, 2 mM folinic acid, 5 mM dTDP-Qui4N, 200 mM NaCl, 100 mM HEPES |
