6NBM
CRYSTAL STRUCTURE OF ENOLASE FROM LEGIONELLA PNEUMOPHILA BOUND TO PHOSPHATE AND MAGNESIUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 116.770, 116.770, 142.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.190 - 1.900 |
R-factor | 0.163 |
Rwork | 0.161 |
R-free | 0.19500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nb2 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (DEV_3318) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.051 | 0.529 |
Number of reflections | 77934 | 31590 |
<I/σ(I)> | 20.1 | 3.07 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 13.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | MORPHEUS H3 (50 MM IMIDAZOLE, 50 MM MES PH 6.5, 20 MM L-GLUTAMIC ACID, 20 MM ALANINE, 20 MM GLYCINE, 20 MM LYSINE;, 20 MM SERINE, 20% GLYCEROL, 10% PEG 4000, TRAY 297444H3 PUCK RLK3-10 |